FD Rapid GolgiStain készlet (nagy)
| termék név | Katalógusszám | Méret | Letöltések | Technikai segítségnyújtás | |
| FD Rapid GolgiStain ™ készlet (nagy) | PK401 | Akár 50 egéragy |  KézihivatkozásokMSDS |
Videó Protocol / Papír |
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Az 1, 2 Golgi-Cox impregnálás az egyik leghatékonyabb technika az idegsejtek normális és rendellenes morfológiájának, valamint a glia tanulmányozásának. A Golgi-technika alkalmazásával finom morfológiai változásokat fedeztek fel a neuronális dendritekben és a dendritikus tüskékben a gyógyszerekkel kezelt állatok agyában, valamint a neurológiai betegségekben szenvedő betegek postmortem agyában 3, 4 . A Golgi-festés megbízhatatlansága és időigényes folyamata azonban komoly akadályt jelentett e technika széles körű alkalmazásában.
Az FD Rapid GolgiStain Kit a Ramón-Moliner 2 , Glaser és Van der Loos 5 által leírt módszerek elvén alapul . Ez a készlet nemcsak drámai módon javította és leegyszerűsítette a Golgi-Cox technikát, de rendkívül megbízhatónak és érzékenynek bizonyult az idegsejtek és a glia, különösen a dendrit tüskék morfológiai részleteinek bemutatására is. Az FD Rapid GolgiStain ™ készletet széles körben és széles körben tesztelték számos állatfaj agyán, valamint a halál utáni emberi agyak mintáin.
A készlet tartalma:
Szobahőmérsékleten tárolandó
A-oldat 250 ml
B-oldat 250 ml
C-oldat 250 ml x 2
D-oldat 250 ml
E-oldat 250 ml
Üvegminta-retriever 2
Természetes hajkefe 2
Cseppentő üveg 1
Szükséges anyagok, de nem tartoznak hozzá:
- Kétszer desztillált vagy ioncserélt víz.
- Műanyag vagy üveg csövek vagy injekciós üvegek.
- Szövettani kellékek és felszerelések, beleértve a zselatinnal bevont mikroszkóp tárgylemezeket, fedőlemezeket, festőedényeket, etanol, xilol vagy xilol helyettesítőit, gyantás rögzítő közeget (pl. Permount®) és egy fénymikroszkópot.
Általános leírása
Kényelmesen csomagolt, rendkívül stabil és rendkívül érzékeny ProteoSilver ideális termék minden fehérjetudós számára. A készlet az ezüstfestő reagensek elkészített oldatait és részletes utasításait tartalmazza az optimális eredmények elérése érdekében. A 0,1 ng / mm 2 fehérje (BSA) kimutatási határával és rendkívül alacsony háttérrel a ProteoSilver az alacsony bőségű fehérjék kiváló kimutatásához vezet.
Alkalmazás
A fehérjék vizualizálására szolgáló gélek festésére a ProteoSilver ™ Silver Stain Kit-t használták.
Tulajdonságok és előnyök
- Az előkevert és előre lemért megoldások csökkentik az egyes alkatrészek beszerzésének és előkészítésének idejét és költségeit
- Az optimalizált protokollok segítenek megteremteni a feltételeket a legjobb eredmény elérése érdekében
- A magas érzékenység és az alacsony háttér biztosítja, hogy a fehérjék nagyon alacsony mennyiségben detektálhatók és feloldhatók legyenek más fehérjékből
- MALDI kompatibilis – Az érdekes fehérjefoltokat tovább jellemezhetjük tömegspektrometriával
- A szobahőmérsékleti stabilitás lehetővé teszi a könnyű és kényelmes tárolást
Jogi információk
A ProteoSilver a Sigma-Aldrich Co. LLC védjegye.
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Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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| 97-086 | ProSci | 0.1 mg | EUR 714.3 |
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Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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SARS-CoV-2 (COVID-19) S1 Recombinant Protein |
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| 97-087 | ProSci | 0.1 mg | EUR 752.1 |
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Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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SARS-CoV-2 (COVID-19) S1 Recombinant Protein |
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| 97-092 | ProSci | 0.1 mg | EUR 714.3 |
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Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
|||
SARS-CoV-2 (COVID-19) S1 Recombinant Protein |
|||
| 10-409 | ProSci | 0.1 mg | EUR 714.3 |
|
Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
|||
SARS-CoV-2 (COVID-19) S1 Recombinant Protein |
|||
| 10-422 | ProSci | 0.1 mg | EUR 714.3 |
|
Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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